Double immunofluorescence with antibodies against MIF orange and ED1(CD68), ED2(CD 163) FITC, green, furthermore, DAPI-medium and thereby, visualization of the nuclei in blue. Overlay-imaging with GIMP of the three single shots. Objective magnification 20x. The clear ED-positive macrophages of the testicular interstitium are clearly visible. The edges of the tubuli semniferi are also MIF-positive.
