Creative Biostructure offers from the single package to the full range of membrane protein service, starting from DNA cloning to the determination of protein structure. Collaborating with academic organizations, we have optimized Lipidic Cubic Phase (LCP) crystallization method to increase the speed and chance of success.
LCP crystallization is the most commonly used method for membrane protein structure determination. It has a membrane-mimetic matrix suitable for stabilization and crystallization of membrane proteins in lipidic environment. Recent advances in pre-crystallization assays, improvement in crystal imaging, and commercial availability of LCP instruments greatly attract the attention of structural biologists. LCP crystallization method has evolved over the years from using small glass test tubes and mixing lipid with protein solution by centrifugation to faster and more efficient way of mixing LCP with syringe mixer and setting up crystallization trials in 96-well plates. Consumption of protein and lipid per crystallization trial has been reduced by over 100-fold, and the whole process becomes as easy as setting up vapor diffusion trays.