Creative Biostructure offers high-throughput UniCrys™ X-ray crystallography services by using state-of-the-art facilities, and has developed a crystallography pipeline that includes all the stages from protein expression to structure determination. Our experienced scientists can teamwork with customers and accelerate the research progress.
After successful crystallization and the diffraction pattern is obtained with our protein crystallization services, the next challenge is to get an electron density map from which detailed information about the structure can be deduced. This is only possible if the ‘phase problem’ can be solved. Each reflection on the diffraction pattern corresponds to a wave consisting of an amplitude and a phase. The amplitude of diffraction spots can be simply measured by taking the square root of the intensity that X-ray detector records. However, the information on the phases, which contain critical information for determining the electron density distribution in the unit cell, is lost during data collection.