If your research project needs to study interactions between specific proteins or modified forms of proteins and a genomic DNA region, for example, to determine whether a transcription factor interacts with a candidate target gene, or monitor the presence of histones with post-translational modifications at specific genomic locations, Creative BioMart provides our customers with state-of-art ChIP-based services to ensure your research needs are met.
What Is ChIP?
Chromatin immunoprecipitation (ChIP) is an antibody-based, large-scale technique used to enrich and capture target protein-DNA complexes. Following the ChIP, protein-DNA interactions can be analyzed using multiple downstream applications, including ChIP-Seq, ChIP-qPCR and ChIP-on-chip. Standard ChIP procedure includes cross-linking protein-DNA complexes in cells, extracting and shearing chromatin, preclearing to reduce non-specific immunoprecipitation, followed by immunoprecipitation with specific antibodies, elution of the protein-DNA complexes from resin, reverse the covalent cross-links, and then the DNA fragments are purified and analyzed by downstream applications.