The events of meiosis I in a living insect spermatocyte beginning at metaphase with meiotic bivalents at the spindle equator followed by anaphase , segregation of bivalents to the spindle poles and the near completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 60 sec over 90 min by a QImaging Retiga EXi CCD camera. A movie prepared from this image stack is included in the grouped set.
Biological Process: Spindle assembly involved in male meiosis II
Author: James R. LaFountain
Source: The Cell: An Image Library
