The events of meiosis I in a living insect spermatocyte beginning at metaphase I with bivalents at the spindle equator followed by anaphase, segregation of half-bivalents diakinesis and commencement of cytokinesis. Time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 60 sec over 105 min by a QImaging Retigo EXi CCD camera. Raw images were processed using 4-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).
Biological Process: Spindle assembly involved in male meiosis II
Author: James R. LaFountain
Source: The Cell: An Image Library
