Visualization of clathrin coated pit dynamics. Total internal reflection fluorescencec microscopy (TIRF-FM) was performed on live human retinal pigment epithelial (RPE) cells stably expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) using a 100x 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon) coupled to a 14-bit mode operated Hamamatsu Orca II-ERG camera. This single channel TIR-FM movie, showing clathrin coated pit dynamics at the ventral plasma membrane, was acquired at a frame rate of 1s with a 70 msec exposure time during 10 min. Pixel size: 67 nm
Biological Process: Clathrin coated pit dynamics
Author: Sandra L. Schmid
Source: The Cell: An Image Library
