Meiotic newt oocyte nuclei were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 10 KX. This micrograph, showing transcription on looped lampbrush chromosomes was recorded at a tilt angle of 45 degrees. The image is grouped with one of the same sample area recorded at a 55 degree tilt. A stereo examination of the pair provides an oblique 3D view.
Biological Process: Transcription, RNA metabolic process
Author: Hans Ris
Source: The Cell: An Image Library