A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc20, a cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C) required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate novel protein thought to be involved in cell cycle regulation. These phase, gfp z-series images are part of an image group that ranges from CIL:39521-39525. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
Biological Process: Activation of mitotic anaphase-promoting complex activity, Mitotic metaphase/anaphase transition, Mitotic sister chromatid segregation, Mitotic spindle elongation, Mannoprotein catabolic process
The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please see the microscopy section in the referenced manuscript for details for image analysis.
Author: Jean Peccoud
Source: The Cell: An Image Library