A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgDLC (dynein light chain). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Raw data stack corresponds to deconvolved stack: CIL# 10492.
Unpublished image that is similar to images published in PMID: 16518471.
See publication for other experimental details.
Biological Process: Regulation of cell shape, Microtubule-based movement
Author: John Murray (University of Pennsylvania)
Source: The Cell: An Image Library