An image of the nucleoli of the macronucleus. Nucleoli have a core of DNA that contains the genes that are transcribed into ribosomal RNA. Assembly of the ribosomal subunits from this processed RNA and from proteins transported into the nucleus through the pores occurs in the nucleoli. A concentric stack of rough ER encircles a mitochondrion. TEM taken on 8/16/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. Bar = 1?m. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34537) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Macronucleus organization, Nucleolus organization
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Author: Richard Allen (University of Hawaii)
Source: The Cell: An Image Library