The nuclear envelope of both the macronucleus, seen here, and the micronucleus consists of 2 membranes containing numerous perforations called pores. Proteins are imported through these pores and ribosomal subunits are transported out of the macronucleus through the same pores. TEM taken on 8/16/67 by R. Allen with a Philips 200 operating at 60kV. Neg. 12,400X. Bar = 0.5?m. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34536) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Macronucleus organization, Nuclear membrane organization
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Author: Richard Allen (University of Hawaii)
Source: The Cell: An Image Library