Colpoda is a soil ciliate and this micrograph is characterized by numerous mucocysts and food vacuoles containing bacteria in various stages of digestion. This electron micrograph is near the posterior end of the cell and contains spongiome tubules of the contractile vacuole. Rows of cilia cover the cell and basal body/cilium complexes are present as dikinetids, two complexes in each cortical unit. TEM taken on 5/31/69 by R. Allen with Philips 300 operating at 60kV. Neg. 5,000X. Bar = 1?m. The negative was printed to paper and the image was scanned with a flatbed scanner and the 1?m bar added in Photoshop. This digitized image is available for qualitative analysis. A high resolution image is available in this library (CIL:38831) and can be used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Cytoplasmic organization, Cellular response to food
Author: Richard Allen
Source: The Cell: An Image Library