Oblique section of the Coleps contractile vacuole pore revealing the supporting microtubules and adjacent fibrous layer. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 3/4/69 by R. Allen with Philips 300. Neg. 25,100X. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:9255) is available for quantitative analysis.
Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Water homeostasis, Microtubule cytoskeleton organization, Microfibril organization
Author: Richard Allen
Source: The Cell: An Image Library