Cross section of a Coleps cilium showing the axoneme consisting of 9 doublet and 2 central microtubules. Doublets have a complete A subfiber and an attached B subfiber. Two dynein arms, outer and inner, extend from the A subfiber. The ciliary membrane with a dense external coat surrounds the ciliary axoneme. The plasma membrane has a glycocalyx composed of the dense coat plus longer filaments. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/28/69 by R. Allen with Philips 300 operating at 60kV. Neg. 92,500X. The negative was printed to paper and the image was scanned with a flatbed scanner and 100nm bar added in Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2871) is in the library and available for quantitative analysis.
Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Ciliary or flagellar motility, Protein galactosylation at cell surface
Author: Richard Allen
Source: The Cell: An Image Library