A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. This image is the maximum intensity projection obtained from the deconvolved stack: CIL# 10546 and corresponds with raw data stack: CIL# 10545 and raw data stack with DIC: CIL# 10542.
Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
Biological Process: Regulation of cell shape, Microtubule cytoskeleton organization, Cell division
Author: Ke Hu (Indiana University, Bloomington)
Source: The Cell: An Image Library