gpdh assay
Glycerol-3-phosphate dehydrogenase (NAD+) (GPDH; EC 1.1.1.8) catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and L-glycerol-3-phosphate (G3P) using NAD as a cofactor. This enzyme widely exists in mammalians, eukaryotes and prokaryotes, and it participates in glycerophospholipid metabolism. It is important for its direct responsibility of maintaining the NAD/NADH balance in the glycosome by the reoxidation of the NADH produced by glyceraldehyde-3-phosphate dehydrogenase during glycolysis. The generated G3P is then transported across the glycosomal membrane and reoxidized via the G3P: DHAP shuttle that functions in conjunction with a mitochondrial glycerol-3-phosphate oxidase. In yeasts, the enzyme is also involved in the superpathway of phosphatidate biosynthesis. Therefore, gpdh assay has a promising prospective in pharmaceutical, biotechnical, and nutritional applications. https://www.creative-enzymes.com/service/Enzyme-Activity-Measurement-Of-Glycerol3Phosphate-Dehydrogenase-NAD-Using-Spectrophotometric-Assays_27.html