This study of the zebrafish eye is a series of four images taken at increasing magnifications. The first image is the SEM block face of the zebrafish eye. This is followed by a second image that is a higher magnification TEM image of the plexiform layer and the outer nuclear layer of the zebrafish retina. The third image is a 15,000X TEM image of the bipolar and horizontal cells interdigitating in the OPL layer of the zebrafish retina. This image, the fourth in the series, is a higher magnification (60,000X) of the dense synaptic bar and synaptic vesicles.
Synaptic vesicles (V) surround the dense bar (DB) in the synaptic ribbon complex in the outer plexiform layer of the larval zebrafish retina. 10 dpf Image collected on a JOEL 1230 at 80 kV using a Gatan 967 slow-scan, cooled CCD camera at x60,000. 70 nm thick section.
Microwave processing, osmium tetroxoxide/osmid procedure for enhancing membranes, and copper lead en bloc staining.
Procedure for specimen preparation available at http://www.stanford.edu/~redhair/JoAnn_Buchanan.
Biological Process: Synapse organization, Synaptic transmission
Author: JoAnn Buchanan (Stanford)
Source: The Cell: An Image Library