High resolution micrograph through an oblique section of the cortex of Coleps showing many ribosomes, ordered into whorls and spirals, collectively called polysomes, on the surface of the rough endoplasmic reticulum. Mitochondria with tubular cristae and small discoidal vesicles thought to be endosomes are seen. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/23/69 by R. Allen with Philips 300 operating at 60kV.. Neg. 18,500X. The raw film was scanned with a Nikon Coolscan 9000ED. This high resolution image is suitable for quantitative analysis.
Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Polysome complexes, Vesicle targeting, rough ER to cis-Golgi
Author: Richard Allen
Source: The Cell: An Image Library