Cultured retinal pigment epithelial cell were stably transfected with mCherry-clathrin light chain and EGFP-epsin. Live-cell time lapse images were collected on a Nikon TiE TIRF with a 100x1.49 NA objective (~67nm/pixel) using 120 ms exposures. This single dual color image part of an image series that contains the time lapse for the mCherry-clathrin light chain and the EGFP-epsin movies as separate files.
Author: Sandra L. Schmid
Source: The Cell: An Image Library