Like other peritrichs the pellicle of Vorticella convallaria is sculpted into ridges and grooves that circle the cell perpendicular to the aboral/adoral axis of the cell body. Pellicular pores penetrate the pellicle as cylindrical indentations of the plasma membrane that end as clathrin-coated pits. A system of alveoli underlies the plasma membrane and rod-like densities occupy the cytosolic tips of the pellicular ridges. A fibrous epiplasm covers the inside of the alveoli. Distinct myonemal bands lie in the aboral to adoral direction to the inside of the epiplasm. These bands are associated with the endoplasmic reticulum by unique specializations of the ER. In this EM preparation the ER has artifactually segmented into vesicles caused by the fixation process. Mitochondria occupy the space between the bands. TEM taken on 4/15/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 9,250X. Bar = 1?m. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39464) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Cortical cytoskeleton organization
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Author: Richard Allen (University of Hawaii)
Source: The Cell: An Image Library