Grazing section through an accumulation of uncoated cupulas in the process of attachment to and fusion with a food vacuole membrane. The small round pores apparently represent early stages of fusion of cupulas with the food vacuole membrane. TEM taken on 3/7/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.5?m. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39459) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Golgi to vacuole transport
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Author: Richard Allen (University of Hawaii)
Source: The Cell: An Image Library