A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc6. Cdc 6 is an essential ATP-binding protein required for DNA replication, component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p. These phase, gfp z-series images are part of an image group that ranges from CIL:39531-39535. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
Biological Process: G1/S transition of mitotic cell cycle, Pre-replicative complex assembly, Regulation of chromatin silencing at telomere, Regulation of DNA-dependent DNA replication initiation, Mannoprotein catabolic process
The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please see the microscopy section in the referenced manuscript for details for image analysis.
Author: Jean Peccoud
Source: The Cell: An Image Library