A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged Tgbeta3-tubulin. 50 timepoints in which daughter formation is visible. Images overlaid with DIC. Images acquired using API DeltaVision on an Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: GFP, ex 450-490 em 500-540. Captured with CoolSnapHQ/ICX285. 3D stack is raw data corresponding to deconvolved stack: CIL# 10489.
Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
Biological Process: Regulation of cell shape, Microtubule cytoskeleton organization, Cell division
Author: John Murray (University of Pennsylvania)
Source: The Cell: An Image Library