Individual Mouse Aortic Endothelial Cell (MAEC) migrating in a 3-dimensional collagen gel (1.4mg/mL). Imaged on a spinning disk confocal microscope using a 100x oil immersion objective lens on a Nikon Eclipse Ti equipped with Perfect Focus System, an electronic shutter (Smart shutter, Sutter Instruments; Novato, CA) for transmitted illumination, a linear-encoded X, Y, Z robotic stage with a piezo-driven Z-axis top plate (ASI Technologies, Montgomeryville, PA) and a Yokogawa CSU-X spinning disk confocal scan head in an electronic filterwheel. Total imaging time = 1hr, 12mins.
Biological Process: Cell migration
Author: Clare M. Waterman
Source: The Cell: An Image Library