The radial arms of the Contractile Vacuole Complex or CVC each contain a collecting canal enclosed by a microtubular ribbon split lengthwise into several smaller ribbons, a meshwork of smooth spongiome and, peripheral to this, the bundles of tubules of the decorated spongiome. The microtubules along the collecting canal are immunogold-labeled with anti-b-tubulin as are some single microtubules in the area. TEM taken on 7/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 7,900X. Bar = 1?m. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100?C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:36689) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Contractile vacuole organization, Cytoplasmic microtubule organization
Author: Richard Allen (University of Hawaii)
Source: The Cell: An Image Library