The CV membrane has collapsed into regularly spaced tubules oriented perpendicular to the microtubular ribbon in this ruptured cell. Some tubules and some nearby vesicles contain electron opaque material probably obtained from the mixture of the external medium and cytosolic contents as the CV expelled its content and medium had a chance to enter the CV by backflow. TEM taken on 8/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2?m. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13115) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Author: Richard Allen (University of Hawaii)
Source: The Cell: An Image Library