A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. This is a maximum intensity projection from z slices 10-13 from deconvolved data stack: CIL# 10539. Maximum intensity projection from z slices 3-6: CIL# 10541. Corresponding raw data stack: CIL# 10538 and raw data stack with DIC: CIL# 10543.
Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
Biological Process: Regulation of cell shape, Microtubule cytoskeleton organization, Cell division
Author: Ke Hu (Indiana University, Bloomington)
Source: The Cell: An Image Library